This article is a quick review of the most popular Ozone Therapy
                Application Methods. If you are curious as to how to use ozone,
                here is a list of the most popular ways to use it (step by step).
              The following article was written with specific reference to
                the International Ozone Therapy Standards and according to International
                Ozone Therapy experts is a record of the safest and most effective
                way to perform these application methods.
                
                Before Attempting any of these Ozone Therapy Protocols please
                read this:
                
                1/ You MUST ensure that the ozone generator you are using was
                designed to produce very pure ozone that is free from any contaminants.
                Many manufacturers will claim that their ozone generators are
                suitable for "Medical Ozone Therapy". Unfortunately it is usually
                not true. It is extremely important that you make sure you have
                an ozone generator that produces ozone, without the ozone touching
                any metal, rubber, or plastic while it is inside the ozone generator.
                These materials are broken down (decompose) due to the ozone
                and the byproducts, which are added to the ozone, are toxic.
                Even 'stainless steel', touted to be ozone resistant is actually NOT ozone resistant, but is actually slowly degraded by the ozone and releases the metals that make up the structure of 'stainless steel' such as iron, chromium, molybdenum, magnesium, etc. 
                
                Even GE(General Electric) states on their website that: 
                
                Stainless Steel is a slightly misleading name. A more accurate description would be Harder to Stain Steel. The largest single component of stainless steel is steel. Steel does and will rust. The chromium in "stainless steel" when exposed to oxygen in the atmosphere forms a thin invisible layer called chromium oxide. This invisible layer covering the entire surface gives stainless steel its ability to resist stains and rust. If this layer is damaged (by oxidation) rust is formed on the surface at the point of that damage.
                
                They go on to say: 
                
                Avoid damaging the chromium oxide layer by avoiding the use of cleaners that contain chlorides (fluorine, chlorine, bromine, iodine, etc.).
                
                These Chlorides are "oxidants" and damage the stainless steel through oxidation. It just so happens that Ozone is 35 times more oxidative than chlorine. So if GE is warning you to not allow chlorine to contact the stainless steel because it will oxidize, then think of the damage done to the stainless steel by "Ozone"!! The "Stainless" steel is oxidized releasing chroimum, iron oxide (rust), molybdenum, magnesium and other metals into the ozone gas stream (and into you!). This is why the experts at the ISCO3.org have stated that "The best material to make these electrodes out of is Quartz Glass".
                
                
                Consumers should be
                especially wary of ozone generators that use "ceramic" electrodes. While ceramic could be considered an ozone resistant material, this material (ceramic) needs to be sealed at the end of the electrode in order to ensure the ozone does not leak out. Since it is impossible to seal a ceramic surface to another ceramic surface, most manufacturers will use simple plastic caps on the end of the electrode while others will seal the ends with simple bathroom sealant, both of which will produce toxic byproducts when they come into contact with the ozone. Also, virtually all ceramic electrodes allow the ozone to come into a metal surface (usually the outer wall of the electrode) that the manufacturer will not tell you about. Read more  about the hazards of ceramic electrodes here. 
                
                Never use an Air Purifier or simple little ozone generator made
                for washing vegetables to perform these ozone therapy protocols.
                
                2/ You must make sure that the ozone generator makes the ozone
                from pure oxygen, not air. If there is no oxygen tank with your
                ozone generator, then don't use it. The ozone won't be the proper
                concentration and the ozone will not be pure enough to use for
                Ozone Therapy.
                
                3/ You need to be able to adjust the ozone output concentration
                of the ozone, or the 'strength' of the ozone, because these Ozone
                Therapy Protocols all use slightly different ozone concentrations.
                If you can't adjust the ozone concentration of the ozone generator,
                or if you don't have a chart that shows you the concentrations
                of ozone that are being produced then don't use it for these
                protocols.
              4/ Please
                  Note: It is noted in the literature that all individuals using
                  ozone
                  therapy
                  should also ensure adequate amounts of Vitamins
                B (complex), C, E, A, (& Beta Carotene), Zinc, Selenium,
                and some have recommended N-acetyl-Cysteine, and Copper. Please
                ensure that you take these supplements either a minimum of 6
                hours before using ozone, or at least 1 hour after using ozone).
                
                Rectal Insufflation: Two Methods
                
                As the name suggests, “Rectal Insufflation” refers
                to introducing ozone into the body through the rectum. If you
                are new to ozone, this sounds like a very strange thing to do.
                However, oxygen (O2) has been infused rectally during surgery
                for years, due to the fact that oxygen is easily absorbed through
                the walls of the colon. The same is true for ozone (O3). Rectal
                Ozone Insufflation is performed regularly in clinics worldwide,
                and can also be performed in the home. There are many advantages
                to using Rectal Ozone Insufflation over all other ozone administration
                methods:
              1/ Ozone experts around
                the world* believe that Rectal Ozone Insufflation is "95% as effective as Major Autohemotherapy",
                which is the most accepted ‘blood’ method of introducing
                ozone into the body. (*Renate Viebahn (Germany), Dr. Robert Rowen
                (USA), Dr. Frank Shallenberger MD (USA), Silvia Menendez (Cuba).
                
                2/ Rectal insufflation is painless and a minimally invasive way
                to use ozone in your home that is just as effective as the blood
                methods but requires no needles, no syringes, no discomfort.
                
                3/ Rectal Insufflation requires only 2 to 4 minutes of your time
                whereas the Major Autohemotherapy (the blood method) requires
                up to 40 minutes to complete.
                
                4/ Rectal Insufflation can be performed by the average ‘home
                owner’ and eliminates the need of the patient to constantly
                visit the physician. (It is highly recommended that you seek
                the guidance and professional care of an Ozone Doctor, who can
                assess your situation, prescribe suitable therapies, and monitor
                your condition as you take part in the prescribed therapies).
                
                5/ In years past, it was considered acceptable in North America for Rectal Ozone Insufflation to be performed by feeding the ozone directly from the ozone generator. However, North Americans are slowly catching up to the rest of the world, where this method of Rectal Insufflation (feeding the ozone directly from the ozone generator) is prohibited. Now the preferred method of Rectal Insufflation is to fill a specially designed bag, or a syringe, with the ozone, and then deliver the ozone from the bag or syringe. Both of these methods are discussed here for your reference, but ozone therapy experts will encourage you to use the bag or syringe method because it is far more comfortable, and far safer.
              
                Rectal Insufflation Method 1: Using a Bag or a Syringe:
                  Infusing the ozone straight from the ozone generator has fallen into disfavor over the past few years, as experience and popularity of using a bag or syringe has risen rapidly. If you take ozone directly from the ozone generator for the insufflation you can’t stop the ozone flow or slow it down during the insufflation because the ozone flow is determined by the flow setting on the oxygen regulator (on the oxygen tank). However if you take the ozone from a bag or syringe you have full control over the speed that the ozone enters, and therefore have a much safer and more comfortable experience.
                  
Filling a Bag or Syringe with ozone from the ozone generator, and then using the bag or syringe to deliver the ozone for the insufflation is therefore now the preferred way of using ozone for Rectal Insufflation. The Bag or Syringe method both allow you to slow down or even stop the ozone flow if you start to feel uncomfortable. Wait for the uncomfortable feeling to pass and you can then continue to deliver the ozone at your own speed. Both the syringe and bag method allow the patient to deliver the ozone themselves in the doctor’s office. The bag method has the added benefit of enabling the patient to take a bag of ozone home with them and infuse it there instead of at the office, whereas the doctor would normally prefer that you leave the expensive syringes in the office.
How is this performed? Read on….
1. Attach a 3 way valve to the output tube of your ozone generator, and an ozone destruct to the valve to collect and destroy any offgassing ozone. (The 3 way valve is often called a ‘syringe port’, ‘luer lock’, or a ‘stopcock’. 
              
              2. Refer to the ozone output chart for your ozone generator so that you know the proper oxygen flow settings and the dial setting on your ozone generator (if available). While referring to the ozone chart that accompanied your ozone generator, set the oxygen flow regulator to the proper setting (usually 1/8 or Ľ Liter Per Minute), and if there is a dial on the ozone generator, set it at the proper level to create 30 – 45 ug/ml of ozone (or 30 – 45 “gamma”). Note: If the manufacturer of your ozone generator did not provide you with an ozone production chart (a chart showing the concentrations of ozone that your ozone generator produces), then return the ozone generator for a refund. Do not use an ozone generator unless is produces pure ozone, and unless you know exactly the ozone concentrations it produces.
                
                  
                  3. Turn on the ozone generator. If you have attached your 3 way valve and ozone destruct properly then you should not smell any ozone (it will be destroyed by the ozone destruct).
                  
                  
                  4. 
                  Attach the bag (or the syringe) to the 3 way stopcock.
                Turn the valve on the 3 way stopcock to direct the ozone into the bag (or the syringe).
                Allow the ozone to flow until you have obtained the desired volume of ozone in the bag (or until the syringe is full if you are using a syringe). For the bag filling:
                If you use an oxygen flow setting of 1/8 Liter Per let the ozone flow into the bag for 1 minute to collect 125 cc of ozone (perfect for beginners), or 2 minutes to collect 250 cc of ozone. 
                If you use an oxygen flow setting of 1/4 Liter Per let the ozone flow into the bag for 30 seconds to collect 125 cc of ozone (perfect for beginners), or 1 minute to collect 250 cc of ozone.
                
                5.
Turn the 3 way stopcock back to the original position that will send the ozone that is being created to the ozone destruct again.
                Disconnect the bag or syringe from the 3 way stopcock (if you are using a bag, clamp the line to the bag first; if you are using a syringe, hold the syringe upright to ensure you don’t lose any ozone). 
                
                
                6. 
                Lubricate the catheter with olive oil.
                Insert the catheter 3 – 4 inches.
                Attach the tube (catheter) to the bag, or to the syringe.
                
                7.
If you are using a bag, release the clamp on the hose that goes to the bag. Slowly and gently push the ozone in by squeezing the bag like a tube of toothpaste, or by pushing slowly on the plunger of the syringe.
                If you feel any cramping or discomfort, slow or stop the process until the discomfort resolves itself (most people will feel no discomfort at all).
                When you have infused the ozone, remove the catheter and discard it. (Note: Some websites recommend you clean the catheter for future use, we recommend you discard the catheter to ensure sterility. It is possible to clean and keep the catheter for future use, but you must ensure that you remove all material from the catheter and sterilize it. Never use a catheter more than 5 times because the ozone will begin to destroy the catheter and release toxic oxidized plastic byproducts.)
                Turn off the ozone generator.
                Turn off the oxygen flow valve (regulator).
                Turn off the oxygen tank. 
               
              Rectal
                Insufflation Method 2: Directly from the Ozone Generator (Note: this is provided for your information only; it is recommended that you do NOT use this method): 
              1/ Make sure your oxygen tank is full of oxygen. Most companies
                send oxygen tanks that are empty. If your oxygen tank is not
                full, check your yellow pages for a company that can fill it
                for you.
              2/ If your oxygen regulator is not yet on your oxygen tank,
                attach it now. When you have completed this task make sure your
                oxygen regulator is attached securely to your oxygen tank. Keep
                in mind that you are dealing with a high pressure gas (the oxygen)
                so if you don't have the regulator attached properly, it will
                leak when you turn the oxygen on. If it leaks, you will hear
                a 'hissing' sound. Don't turn on your oxygen flow yet.
              3/ Attach the oxygen line from your oxygen regulator to your
                ozone generator. Various companies use different types of connectors
                to make this connection. If you have purchased your oxygen tank
                from one company, and the ozone generator from another company,
                make sure you can connect the two together (the seal must be
                tight to prevent leaking). Make sure you connect the oxygen to
                the oxygen 'in' and not the ozone 'out' connection.
              4/ Attach tubing to the ozone output of the ozone generator
                so you can get a comfortable distance away from the ozone generator.
                The tubing you are using should be a 'whitish' color and made
                of 'silicone'. Silicone tubing must be used because it won't
                be destroyed by the ozone. Do not use rubber, plastic, or latex
                tubing because they will be destroyed by the ozone.
              5/ Now it's time to set the ozone generator. Refer to the ozone
                chart or graph that you received with your ozone generator. This
                is the guide that they should have provided to you that shows
                you how much ozone the ozone generator produces. If you don't
                have one, ask the manufacturer to send you one. (If they don't
                have one, don't use your ozone generator for any Ozone Therapy
                protocols. You must know what the concentration of the ozone
                is that the ozone generator is producing and you won't unless
                you have a chart or graph). 
              6/ Look at the ozone output guide, graph, or chart to find out
                where 30 - 45 ug/ml of ozone will be produced. Hopefully it will
                be at a flow rate of around 1/8 or 1/4 Liters Per Minute (LPM)
                of oxygen as these are the easiest flow rates to use. (Don't
                turn on the oxygen yet).
              7/ If your ozone generator
                  has a "energy" selector
                on it, or a dial, and make note of the dial to the setting that
                will produce 30 - 45 ug/ml of ozone. Set the dial to this setting
                now, but don't turn on your ozone generator yet. (The dial setting
                information should be displayed on your Ozone Output Chart or
                Graph. If you don't have a dial on your ozone generator, don't
                worry. Just make sure that the 'flow rate' that you have set
                in step 6 will product the proper ozone concentration of 30 -
                45 ug/ml). 
              8/ Take your catheter and have a look at it. One end will have
                holes in it and a rounded tip. This is the end that you are going
                to insert rectally. The other end will have a connector on it,
                this is the end that you connect to the ozone tubing that comes
                from the ozone generator. Make sure that this connection will
                form a tight seal, because if it does not, the ozone will simply
                leak at this connection instead of going into you. If the seal
                is not tight, call the company you purchased the equipment from
                for advice. Some companies provide a 'catheter adapter' that
                will make sure the seal is tight. If you have one, use it.
              9/ Lubricate 3 to 4 inches of the end you are going to insert
                with olive oil. (Do not use any petroleum jelly or other petroleum
                lubrication products!). Inser the tube 3 - 4 inches slowly and
                carefully. If you feel any pain or discomfort, stop. DO NOT connect
                the catheter yet to the ozone generator.
              10/ Now you are ready to start the Rectal Insufflation:
              (a) You will first need to start the flow of oxygen from the
                oxygen tank. To do this you will need to open two valves. The
                first valve is on the top of the tank. Open this valve first
                by turning it counter clockwise as you look at it from above,
                just like opening a jar or bottle. Next, open the other valve
                which is usually the flow selector valve and set it for either
                1/8 or 1/4 LPM.
                (b) Turn on your ozone generator. When you do this, you will
                be making ozone.
                (c) You need to make sure that ozone is coming out of the end
                of the tubing before you connect the tubing to the catheter.
                Most people will wait until they smell ozone coming from the
                end of the tubing. If you are going to do this hold the end of
                the tubing well away from your nose and mouth, and take small
                'snifs'. Ozone can be very irritating if you inhale a lot of
                it.
                (d) When you smell ozone, look at the second hand of your watch
                or a clock and connect the tube to the catheter. When you do
                this, ozone will be flowing into the catheter (and into you!).
                You need to 'time' the whole process so make sure you make note
                of the position of the second hand.
                (e) If you are using 1/8 LPM setting on the oxygen regulator,
                let the ozone flow into the catheter for 1 minute. If you are
                using 1/4 LPM on the oxygen regulator, let the ozone flow  for
                30 seconds.
                (f) Once the time is up, remove the catheter.
                (g) Disconnect the cathter from the tubing.
                (h) Turn off the ozone generator.
                (i) Turn off the oxygen flow.
                (j) Discard the catheter or clean it for another use. You can
                only use a catheter a maximum of 5 times.
              
              Discussion:
                
                (1) How Long do I have to hold the ozone?
                The answer? You don't have to hold the ozone. Cuban scientists have recently proven that the ozone is completely absorbed and reacts with the tissue in the colon immediately upon entry into the rectum and colon. Years ago it was thought that you had to hold the ozone for 20 minutes. You may still find this 'holding time' written in instructions all over the internet. The great news is, this has been proven to be NOT TRUE. If you need to let the ozone go shortly after you have infused it, let it go. You don't have to do the insufflation again because the only thing you are releasing is oxygen, not ozone. As already stated, the ozone component of the gas was immediately absorbed by the body.
              (2) When do I Do This? 
                Any time of day.Perform the procedure after a recent bowel movement.
                  Some people may prefer to perform an enema or colonic before
                  the insufflation, however this is not absolutely necessary.
              (3) How Often? Never more than once a day, 1 to 5 times per
                week.
              (4) What Concentration or Strength?
  You must choose an ozone concentration setting on your ozone
                  generator of 30 - 45 ug/ml or 'micrograms per milliliter'. "Micrograms
                  per milliliter" or "ug/ml" are the concentration
                  terms used to discuss the strength of ozone that should be
                  used for each ozone application method. You must ensure that
                  you can control the ozone concentratration (or 'strength')
                  produced by your ozone generator. Carefully review your ozone
                  generator manual to discover how to do this. If you did not
                  receive a manual with your ozone generator, if the manual does
                  not provide you with information regarding the ozone output
                  levels of your ozone generator, do not perform the insufflation.
                  You must know the concentration of ozone that you are using,
                  and this is only possible if the manufacturer has provided
                  you with an Ozone Output guide.
                  
                  (5) Can I Use and Air Fed Ozone Generator?
                  NO. Air fed ozone generators are for air purification only,
                  and sometimes for light water purification. Air fed ozone generators
                  are 'industrial' ozone generators. The ozone they produce is
                  not pure enough for ozone therapy (the ozone has contaminants
                  in it), and it is not strong enough for ozone therapy. Make
                  sure you are using an ozone generator that requires pure oxygen
                  to be fed into it.
              (6) How Much
                  Ozone? That is, What Volume?
  You must be using an ozone generator that uses pure oxygen as
                  a feed gas. The flow rate of oxygen should be set for 1/4,
      1/8, or 1/16 Liter Per Minute; choose the flow rate that provides you with
                  a concentration of 30 - 45 ug/ml. Never use an ozone generator
                  that uses "air" for this procedure, as the ozone
                  will not be the proper concentration, and the ozone will be
                  contaminated with toxins and byproducts from the air. The speed
                  that the oxygen enters the ozone generator is called the "flow
                  rate" of oxygen. The flow rate will determine the concentration
                  of ozone that the ozone generator produces. The ideal flow
                  rate of oxygen to use for an insufflation is 1/4, 1/8, or 1/16
                  LPM (Liters per Minute), but only if your ozone generator produces
                  30 - 45 ug/ml of ozone at one of these flow rates. This is
                  the reason you need an accurate chart or readout of the ozone
                  concentrations the ozone generator produces. If you do not
                  have a chart that clearly indicates the concentrations of ozone
                  your ozone generator creates, do not perform the insufflation
                  until you have this information.
              Infusing gas rectally is much like blowing up a balloon. For
                safety and comfort reasons the physician and patient must always
                ensure that only the desired volume of ozone gas is infused,
                at the desired concentration of 30 - 45 ug/ml. As described above,
                a typical starting point for many users is 125cc of gas. The
                ozone gas is infused through the catheter which is inserted into
                the rectum approximately 3 to 4 inches. Using the example above,
                the flow is then stopped, the catheter withdrawn.
              (7) Can I
                use Higher Concentrations of Ozone?
              Only for a specific purpose (Bleeding Colitis). If your goal
                is to use ozone for detoxification and to boost the immune system
                make sure you are using 30 - 45 ug/ml. Do not increase the ozone
                concentration because you will not be increasing the effectiveness
                and will only cause irritation.
              The only time this
                  technique varies is if the desired outcome of the rectal insufflation
                  is to stop the bleeding associated
                with "Bleeding Colitis". In this case, the German standards
                indicate that the concentration of ozone should be 60ug/ml and
                the amount of ozone infused should only be 60cc. This higher
                ozone concentration will stop the bleeding, but will not heal
                the colon. Therefore the goal is to first stop the bleeding with
                the higher concentration (60ug/ml) and once stopped, the technique
                above is used to heal the affected parts of the colon. The basic
                guidelines are that ozone at higher concentrations (60 ug/ml)
                are used to stop bleeding and to sterilize. After this is accomplished
                the ozone concentration should be lowered to the levels known
                to cause healing, that is, 30 - 45 ug/ml.
              (8) Can I
                  do this for a longer period of time to increase the effectiveness?
                NO. A recent publication encouraging a "45 minute rectal
                insufflation" should never be attempted. The author of this
                technique is under the impression that "more is better" and
                therefore performing an insufflation for this length of time
                should be preferable to the short rectal insufflation. Unfortunately
                the person who devised this technique and published it in a book
                is not a doctor, and has no previous medical training. He therefore
                has no training or knowledge with regards to how damaging this
                45 minute rectal insufflation would be to people. The guidelines
                instruct someone to use two catheters, one deep inside the colon,
                and one only inserted a few inches. One catheter is used to infuse
                the ozone, while the other is used to allow the ozone to escape.
                This prevents the build up of any pressure within the colon,
                and allows the user to infuse ozone for as long as desired. Again,
                this method is assuming that 'more is better'. Unfortunately
                this logic is flawed and has absolutely no scientific basis.
                There is no increase in benefit in using this method, and in
                fact, the user attempting this method runs a very clear risk
                of massive irritation of the sensitive tissue within the colon.
                Ozone in proper amounts is a wonderful healing tool, however
                attempting a 45 minute rectal insufflation can actually cause
                irritation and damage of this sensitive tissue.
              
                Ozone Sauna 
              The benefits
                  of a steam sauna on the immune system and disease processes
                  have been well documented. In addition to being relaxing and
                  soothing, a sauna and the accompanying induced hyperthermia
                  on the body, mimics the beneficial effects of a fever, without
                  the discomfort. At 104 degrees F., for example, the growth
                  rate of the polio virus is reduced up to 250 times; at 106
                  degrees pneumococcus, a bacterium responsible for pneumonia,
                  dies. 
              Although
                  the effects of this artificial method of increasing the body
                  temperature are not as comprehensive as a natural fever, there
                  are definite system wide effects. There is evidence that artificial
                  fever works as an immune system stimulant by increasing the
                  number of white blood cells in the body. In a 1959-review of
                  studies on the effects of heat treatments, Mayo Clinic researcher
                  Dr. Wakim and colleagues cite findings indicating that the
                  number of white blood cells in the blood increased by an average
                  of 58% during artificially induced fever. Researchers also
                  have found increases in the activity of the white blood cells
                  during induced fever. 
              Apart from
                  the immune system-stimulating effects of sweat therapy, many
                  thought it as one of the most effective and painless detoxifying
                  treatments available. Sweat contains almost the same elements
                  as urine, and for this reason, the skin is sometimes called
                  the third kidney. It is estimated that as much as 30% of bodily
                  wastes are eliminated by way of perspiration, and during a
                  steam sauna, the body perspires profusely. 
              By adding
                  ozone into this environment, the ozone is easily absorbed into
                  the skin and lymphatic system because of the open pores. This
                  provides an excellent detoxifying effect and it is also reported
                  that the effects are once again not limited to detoxification,
                  but may induce the desirable system wide effects that is desired
                  in the medical use of ozone: oxygenation of all the tissues,
                  organs, and cells of the body, boosting the immune system,
                  stimulation of the release of anti-cancer substances from the
                  white blood cells of the immune system, enhancement of blood
                  and oxygen delivery throughout the body. 
              It is very
                  important to point out that the sauna used for this treatment
                  is of the type where the individual's head protrudes from the
                  top of the sauna, and the individual is therefore not breathing
                  the ozone / steam mixture. Concentrations used for this type
                  of treatment are usually approximately 30 - 40ug/ml, and the
                  ozone is introduced into the sauna at a rate of 1/4LPM. 
              
              Using the
                  steam sauna is one of the most popular methods of taking advantage
                  of this therapy. The steam sauna ozone system for applying
                  ozone has many advantages: 
              (1) It is a relaxing
                  and non-invasive approach to ozone therapy 
  (2) It cleanses the skin, pores, and lymphatic system 
  (3) The user will take advantage of 2 therapies at the same time: sweat therapy
  and ozone therapy (both individually with their own health benefits) 
  (4) You feel clean, refreshed, and rejuvenated. 
              Auricular
                  Insufflations: 
              Many individuals
                  have found it beneficial to irrigate the ear canal with the
                  ozone / oxygen mixture from a medical ozone generator. As with
                  every use of ozone for medical purposes the individual must
                  ensure: the purity of the oxygen supply, that all parts of
                  the ozone generator in contact with the gas stream are ozone
                  resistant (eg. kynar, Teflon, glass, silicone), and that they
                  know the ozone output (concentration of the ozone) of the ozone
                  generator. This is extremely important - too little ozone and
                  there will be no effect; too much ozone and there can be irritation
                  to the ear canal. 
              The patient
                  may first apply a small amount of water to the ear canal to
                  assist in the absorption of the ozone. The ozone must be humidified
                  by bubbling the gas through water. The usual concentration
                  used for this protocol is 15ug/ml - 30ug/ml. The flow rate
                  used is usually ¼ Litre per minute or perhaps 1/8 LPM. 
              The output
                  tube of the ozone generator is held up to the entrance of the
                  ear. At no time should the tube come into proximity of the
                  eyes, nose, or mouth. Doing so may cause discomfort. At no
                  time should the output tube be placed inside the ear, or sealed
                  into the entrance way of the ear. 
  The ozone should be allowed to flow into the ear and out of the ear. Each ear
  is usually treated for 2-5 minutes per treatment. 
              Treatment
                  schedule depends on the disease of the patient, and the effect
                  on each individual patient. This therapy should not be performed
                  more than once per day, and in most cases is performed 2-3
                  times per week. 
              Patients
                  should individually modify the treatment protocol depending
                  on their reaction. If any discomfort, redness, or "chapping" occurs,
                  it is suggested that the patient increase the length of time
                  between treatments, and decrease the ozone concentration coming
                  from the ozone generator. 
              Many physicians
                  have indicated that the ozone enters the lymphatic and blood
                  system this way. Many patients have reported a wide variety
                  of results including relief of allergies, relief of colds,
                  clearing of sore throats and swollen glands. 
  Vaginal Insufflation: 
              A vaginal
                  cannula is used to introduce the ozone gas to the patient.
                  Unlike Rectal Insufflation, there is no danger of pressure
                  buildup. The ozone concentration used is usually 25-30ug/ml,
                  and the flow rate is usually 1/4LPM (litres per minute) or
                  1/8LPM. Treatment time is usually 5 - 15 minutes. The patient
                  may lower the ozone concentration, and the duration of the
                  treatment if any discomfort occurs. This treatment should not
                  be performed more than once per day, and is usually only performed
                  2-3 times per week. 
              Many women
                  have reported relief from yeast infections and various sexually
                  transmitted diseases including herpes. Many women also use
                  this method as an alternative to rectal insufflation as it
                  is theorized that the ozone not only affects the pelvic region,
                  but also enters the general circulation causing a body wide
                  effect. 
              Women must
                  not use this method close to, or during the time of menstruation
                  as ozone at these concentrations increases blood flow. 
              Body Suit 
              One of the
                  easiest and most pleasurable of the methods of using ozone,
                  the body suit is both effective and relaxing. The ozone is
                  absorbed through the skin, cleansing the lymphatic system,
                  and reportedly inducing the body wide effects of ozone generally
                  seen with other treatment protocols. 
              The patient
                  first opens the pores of the skin by taking a warm/hot shower,
                  after which they immediately enter the body suit. 
  The body suit must be sealed at the ankles and wrists to reduce leaking. Usually
  a towel is wrapped around the neck to increase comfort and to reduce leaking
  around the neck. If necessary, a fan may be used to gently blow any leaking
  ozone away from the individual. The ozone generator is set to produce a concentration
  of 25 - 35ug/ml at a flow rate of 1/4LPM. 
  The ozone must be humidified (run through water) and is introduced into the
  body suit. 
              The patient
                  remains in the body suit for 15 - 40 minutes ( one usually
                  starts with a 15 minute treatment and the treatment time remains
                  that or is gradually increased depending on the desire and
                  comfort of the individual during and after treatments). Treatment
                  is usually individualized depending on the individual effects
                  of the treatments and the desired effect, be it in treatment
                  of a disease, or for general health. Individuals should be
                  encouraged to use their own bodies and common sense as an indication
                  of whether treatment times and concentrations should be increased
                  or lowered. This treatment is usually performed 2-3 times per
                  week, and never more than once per day. 
              Ozonated
                  Olive Oil 
              Ozonated
                  Olive Oil is used around the world for a variety of uses: acne,
                  skin lesions, burns, fungal infections (eg. Of the toe nail),
                  herpes, eczema, leg sores, bed sores, gingivitis, hemorrhoids,
                  STD's, cold sores, and many others. 
              This appears
                  to be the only way to stabilize ozone without adding artificial
                  stabilizers, chemicals, or preservatives. Ozone is bubbled
                  at very high concentrations, under a controlled environment
                  for days until it slowly begins to solidify. This solid form
                  of olive oil forms a Vaseline or salve like substance and will
                  keep for many months on the shelf. If kept refrigerated, it
                  maintains its full effectiveness almost indefinitely. 
              Some individuals
                  even choose to ingest ozonated olive oil in order to obtain
                  the system wide effects of using ozone medically. Click here
                  for instructions as to how to make ozonated olive oil. 
              Bagging 
              "Bagging" with
                  ozone refers to the method of isolating a body part by surrounding
                  it with a bag (such as hand, arm, leg, foot, torso, pelvis,
                  but NEVER head), and introducing ozone. This can be used to
                  treat gangrene, diabetic foot ulcers, bed sores, burns, any
                  wounds that are infected or slow healing, or those that refuse
                  to heal. This method is not intended to generate an immune
                  modulating effect on the individual as in the methods above. 
              The bag is
                  placed around or over the affected area, the output tube from
                  the ozone generator placed through the top of the bag, and
                  the top sealed as effectively as possible. Ozone at the desired
                  concentration must first be humidified (simply bubbled through
                  water) and then enters the bag; the ozone generator constantly
                  runs during this treatment. Treatment times vary depending
                  on the type of wounds being treated but typically range from
                  10 - 30 minutes. As leaking will occur from the bag it is suggested
                  that this process occurs in a well ventilated area. After the
                  treatment the bag will still contain a high concentration of
                  ozone, irritating to the lungs, nose, and eyes if it escapes
                  from the bag. This is usually disposed of in a safe manner.
                  (In European medical clinics, a specially designed bag is used
                  to ensure no leaking occurs, and after treatment the ozone
                  is sucked from the bag by a vacuum pump so neither the patient,
                  nor the attendant ever breathes in the ozone). 
              In this method
                  the ozone kills any bacteria, viruses, fungus, or molds infecting
                  the open wound, increase blood flow to the wound, and stimulates
                  the healing process. It has been documented that many a body
                  part has been spared amputation through the application of
                  ozone in this method. 
              High concentrations
                  of ozone applied through bagging (60ug/ml - 90ug/ml) tend to
                  have a sterilization effect on the wound, but if used for prolonged
                  periods will have a negative effect on healing. Mid range ozone
                  concentrations (30-40 ug/ml) will have a healing effect on
                  the wounds. Therefore the protocol as suggested by German researchers
                  is to begin with the infected wound at 75-90ug/ml for the sterilizing
                  effect, and as the treatments begin to clear the wound of infection,
                  to gradually drop the concentration towards 35ug/ml. This gradual
                  drop in the concentration will maintain the sterility of the
                  wound, and stimulate healing. Individual differences in this
                  protocol are taken into account by watching the effect of the
                  ozone on the wound, and the rate at which it closes. 
              Major Autohemotherapy 
              This procedure
                  is performed only by trained professionals, usually only in
                  a clinic setting. Major Autohemotherapy
                  is the preferred method of many practitioners, and it is used
                  in well over 150 diseases. In Germany, even the ambulances
                  are equipped with ozone generators; if ozone is administered
                  within 24 hours of a stroke, 95% of patients suffer no permanent
                  paralysis.
              The Major
                  Autohemotherapy protocol
                  obviously requires medical grade ozone with no contaminants.
                  Ozone generators that produce ozone from 'air' should never                  be used for this protocol, even if they have been fitted with
                  an oxygen tank. Only ozone generators designed to produce pure
                  medical ozone should be used. This protocol requires standard
                  blood administration equipment, such as a Blood Administration
                  Set
                  (transfusion
                  line), butterfly
                  needles,
                  evacuated container (or empty saline bag), heparin, calcium,
                  and 60 cc syringes. The procedure involves the removal of approximately
                  125 to 200cc of the patients blood. This blood is withdrawn
                  into the evacuated bottle or into an empty saline bag. The
                  bottle (or bag) is then hung upside down, and ozone gas (the
                  same
                  volume as the
                  blood
                  withdrawn) is infused into the blood. But at what concentration?
                  
                  Professor
                  Velio Bocci of Italy can be accredited with being a major influence
                  in
                  the world of ozone in that he has been researching ozone for
                  over 30 years at the University of Sienna. His research has
                  shown
                  that greatest immune system stimulation (activation) effects
                  occur when the ozone is introduced into the blood at a concentration
                  of 78
                  ug/ml.
                  His detailed research has displayed that this concentration
                  of 78 ug/ml illicites the maximum cytokine production from
                  the white blood cells.
                  These cytokines enter the body when the blood is reintroduced
                  to the patient, and in turn stimulate other white blood cells
                  within the body to also release cytokines, inducing an immunostimulating
                  effect that spreads through the body like a chain reaction.
                  While lower concentrations of ozone can be used for Major Autohemotherapy,
                  Bocci's research clearly showed that cytokine production is
                  maximized when the ozone is bubbled through the blood at 78
                  ug/ml.. 
              In this procedure
                  the trained professional constantly monitors the patient, and
                  ensures the purity of the ozone gas, and the sterility of all
                  equipment used. For the trained professional, this treatment
                  is actually quite easy, and very safe for the patient.