This article is a quick review of the most popular Ozone Therapy
Application Methods. If you are curious as to how to use ozone,
here is a list of the most popular ways to use it (step by step).
The following article was written with specific reference to
the International Ozone Therapy Standards and according to International
Ozone Therapy experts is a record of the safest and most effective
way to perform these application methods.
Before Attempting any of these Ozone Therapy Protocols please
1/ You MUST ensure that the ozone generator you are using was
designed to produce very pure ozone that is free from any contaminants.
Many manufacturers will claim that their ozone generators are
suitable for "Medical Ozone Therapy". Unfortunately it is usually
not true. It is extremely important that you make sure you have
an ozone generator that produces ozone, without the ozone touching
any metal, rubber, or plastic while it is inside the ozone generator.
These materials are broken down (decompose) due to the ozone
and the byproducts, which are added to the ozone, are toxic.
Never use an Air Purifier or simple little ozone generator made
for washing vegetables to perform these ozone therapy protocols.
2/ You must make sure that the ozone generator makes the ozone
from pure oxygen, not air. If there is no oxygen tank with your
ozone generator, then don't use it. The ozone won't be the proper
concentration and the ozone will not be pure enough to use for
3/ You need to be able to adjust the ozone output concentration
of the ozone, or the 'strength' of the ozone, because these Ozone
Therapy Protocols all use slightly different ozone concentrations.
If you can't adjust the ozone concentration of the ozone generator,
or if you don't have a chart that shows you the concentrations
of ozone that are being produced then don't use it for these
Note: It is noted in the literature that all individuals using
should also ensure adequate amounts of Vitamins
B (complex), C, E, A, (& Beta Carotene), Zinc, Selenium,
and some have recommended N-acetyl-Cysteine, and Copper. Please
ensure that you take these supplements either a minimum of 6
hours before using ozone, or at least 1 hour after using ozone).
As the name suggests, “Rectal Insufflation” refers
to introducing ozone into the body through the rectum. If you
are new to ozone, this sounds like a very strange thing to do.
However, oxygen (O2) has been infused rectally during surgery
for years, due to the fact that oxygen is easily absorbed through
the walls of the colon. The same is true for ozone (O3). Rectal
Ozone Insufflation is performed regularly in clinics worldwide,
and can also be performed in the home. There are many advantages
to using Rectal Ozone Insufflation over all other ozone administration
1/ Ozone experts around
the world* believe that Rectal Ozone Insufflation is "95% as effective as Major Autohemotherapy",
which is the most accepted ‘blood’ method of introducing
ozone into the body. (*Renate Viebahn (Germany), Dr. Robert Rowen
(USA), Dr. Frank Shallenberger MD (USA), Silvia Menendez (Cuba).
2/ Rectal insufflation is painless and a minimally invasive way
to use ozone in your home that is just as effective as the blood
methods but requires no needles, no syringes, no discomfort.
3/ Rectal Insufflation requires only 2 to 4 minutes of your time
whereas the Major Autohemotherapy (the blood method) requires
up to 40 minutes to complete.
4/ Rectal Insufflation can be performed by the average ‘home
owner’ and eliminates the need of the patient to constantly
visit the physician. (It is highly recommended that you seek
the guidance and professional care of an Ozone Doctor, who can
assess your situation, prescribe suitable therapies, and monitor
your condition as you take part in the prescribed therapies).
Insufflation Method: The instructions for the procedure are provided here.
about some of these instructions read on,
as the "Discussion" section should answer those questions
1/ Make sure your oxygen tank is full of oxygen. Most companies
send oxygen tanks that are empty. If your oxygen tank is not
full, check your yellow pages for a company that can fill it
2/ If your oxygen regulator is not yet on your oxygen tank,
attach it now. When you have completed this task make sure your
oxygen regulator is attached securely to your oxygen tank. Keep
in mind that you are dealing with a high pressure gas (the oxygen)
so if you don't have the regulator attached properly, it will
leak when you turn the oxygen on. If it leaks, you will hear
a 'hissing' sound. Don't turn on your oxygen flow yet.
3/ Attach the oxygen line from your oxygen regulator to your
ozone generator. Various companies use different types of connectors
to make this connection. If you have purchased your oxygen tank
from one company, and the ozone generator from another company,
make sure you can connect the two together (the seal must be
tight to prevent leaking). Make sure you connect the oxygen to
the oxygen 'in' and not the ozone 'out' connection.
4/ Attach tubing to the ozone output of the ozone generator
so you can get a comfortable distance away from the ozone generator.
The tubing you are using should be a 'whitish' color and made
of 'silicone'. Silicone tubing must be used because it won't
be destroyed by the ozone. Do not use rubber, plastic, or latex
tubing because they will be destroyed by the ozone.
5/ Now it's time to set the ozone generator. Refer to the ozone
chart or graph that you received with your ozone generator. This
is the guide that they should have provided to you that shows
you how much ozone the ozone generator produces. If you don't
have one, ask the manufacturer to send you one. (If they don't
have one, don't use your ozone generator for any Ozone Therapy
protocols. You must know what the concentration of the ozone
is that the ozone generator is producing and you won't unless
you have a chart or graph).
6/ Look at the ozone output guide, graph, or chart to find out
where 30 - 45 ug/ml of ozone will be produced. Hopefully it will
be at a flow rate of around 1/8 or 1/4 Liters Per Minute (LPM)
of oxygen as these are the easiest flow rates to use. (Don't
turn on the oxygen yet).
7/ If your ozone generator
has a "energy" selector
on it, or a dial, and make note of the dial to the setting that
will produce 30 - 45 ug/ml of ozone. Set the dial to this setting
now, but don't turn on your ozone generator yet. (The dial setting
information should be displayed on your Ozone Output Chart or
Graph. If you don't have a dial on your ozone generator, don't
worry. Just make sure that the 'flow rate' that you have set
in step 6 will product the proper ozone concentration of 30 -
8/ Take your catheter and have a look at it. One end will have
holes in it and a rounded tip. This is the end that you are going
to insert rectally. The other end will have a connector on it,
this is the end that you connect to the ozone tubing that comes
from the ozone generator. Make sure that this connection will
form a tight seal, because if it does not, the ozone will simply
leak at this connection instead of going into you. If the seal
is not tight, call the company you purchased the equipment from
for advice. Some companies provide a 'catheter adapter' that
will make sure the seal is tight. If you have one, use it.
9/ Lubricate 3 to 4 inches of the end you are going to insert
with olive oil. (Do not use any petroleum jelly or other petroleum
lubrication products!). Inser the tube 3 - 4 inches slowly and
carefully. If you feel any pain or discomfort, stop. DO NOT connect
the catheter yet to the ozone generator.
10/ Now you are ready to start the Rectal Insufflation:
(a) You will first need to start the flow of oxygen from the
oxygen tank. To do this you will need to open two valves. The
first valve is on the top of the tank. Open this valve first
by turning it counter clockwise as you look at it from above,
just like opening a jar or bottle. Next, open the other valve
which is usually the flow selector valve and set it for either
1/8 or 1/4 LPM.
(b) Turn on your ozone generator. When you do this, you will
be making ozone.
(c) You need to make sure that ozone is coming out of the end
of the tubing before you connect the tubing to the catheter.
Most people will wait until they smell ozone coming from the
end of the tubing. If you are going to do this hold the end of
the tubing well away from your nose and mouth, and take small
'snifs'. Ozone can be very irritating if you inhale a lot of
(d) When you smell ozone, look at the second hand of your watch
or a clock and connect the tube to the catheter. When you do
this, ozone will be flowing into the catheter (and into you!).
You need to 'time' the whole process so make sure you make note
of the position of the second hand.
(e) If you are using 1/8 LPM setting on the oxygen regulator,
let the ozone flow into the catheter for 1 minute. If you are
using 1/4 LPM on the oxygen regulator, let the ozone flow for
(f) Once the time is up, remove the catheter.
(g) Disconnect the cathter from the tubing.
(h) Turn off the ozone generator.
(i) Turn off the oxygen flow.
(j) Discard the catheter or clean it for another use. You can
only use a catheter a maximum of 5 times.
(k) Hold the ozone gas inside for at least 20 - 30 minutes. If
you feel comfortable, you can move around during this time.
(1) When do I Do This?
Any time of day.Perform the procedure after a recent bowel movement.
Some people may prefer to perform an enema or colonic before
the insufflation, however this is not absolutely necessary.
(2) How Often? Never more than once a day, 1 to 5 times per
(3) What Concentration or Strength?
You must choose an ozone concentration setting on your ozone
generator of 30 - 45 ug/ml or 'micrograms per milliliter'. "Micrograms
per milliliter" or "ug/ml" are the concentration
terms used to discuss the strength of ozone that should be
used for each ozone application method. You must ensure that
you can control the ozone concentratration (or 'strength')
produced by your ozone generator. Carefully review your ozone
generator manual to discover how to do this. If you did not
receive a manual with your ozone generator, if the manual does
not provide you with information regarding the ozone output
levels of your ozone generator, do not perform the insufflation.
You must know the concentration of ozone that you are using,
and this is only possible if the manufacturer has provided
you with an Ozone Output guide.
(4) Can I Use and Air Fed Ozone Generator?
NO. Air fed ozone generators are for air purification only,
and sometimes for light water purification. Air fed ozone generators
are 'industrial' ozone generators. The ozone they produce is
not pure enough for ozone therapy (the ozone has contaminants
in it), and it is not strong enough for ozone therapy. Make
sure you are using an ozone generator that requires pure oxygen
to be fed into it.
(5) How Much
Ozone? That is, What Volume?
You must be using an ozone generator that uses pure oxygen as
a feed gas. The flow rate of oxygen should be set for 1/4,
1/8, or 1/16 Liter Per Minute; choose the flow rate that provides you with
a concentration of 30 - 45 ug/ml. Never use an ozone generator
that uses "air" for this procedure, as the ozone
will not be the proper concentration, and the ozone will be
contaminated with toxins and byproducts from the air. The speed
that the oxygen enters the ozone generator is called the "flow
rate" of oxygen. The flow rate will determine the concentration
of ozone that the ozone generator produces. The ideal flow
rate of oxygen to use for an insufflation is 1/4, 1/8, or 1/16
LPM (Liters per Minute), but only if your ozone generator produces
30 - 45 ug/ml of ozone at one of these flow rates. This is
the reason you need an accurate chart or readout of the ozone
concentrations the ozone generator produces. If you do not
have a chart that clearly indicates the concentrations of ozone
your ozone generator creates, do not perform the insufflation
until you have this information.
Infusing gas rectally is much like blowing up a balloon. For
safety and comfort reasons the physician and patient must always
ensure that only the desired volume of ozone gas is infused,
at the desired concentration of 30 - 45 ug/ml. As described above,
a typical starting point for many users is 125cc of gas. The
ozone gas is infused through the catheter which is inserted into
the rectum approximately 3 to 4 inches. Using the example above,
the flow is then stopped, the catheter withdrawn, and the patient
then holds this gas for a period of at least 30 minutes.
Why 30 minutes? Dr.
Michael Carpendale has shown us through studies that the ozone
gas is absorbed over a period of 20-30
minutes as it is held in the body. If the ozone escapes before
that time, the full benefit of the treatment has not been obtained.
The gas is slowly absorbed into the body, and the "system
wide" effects of ozone therapy are obtained. Most users
of this method have mentioned that little or no discomfort is
caused by this method, and most find they have no gas to release
at the end of the 30 minute period.
(6) Can I
use Higher Concentrations of Ozone?
Only for a specific purpose (Bleeding Colitis). If your goal
is to use ozone for detoxification and to boost the immune system
make sure you are using 30 - 45 ug/ml. Do not increase the ozone
concentration because you will not be increasing the effectiveness
and will only cause irritation.
The only time this
technique varies is if the desired outcome of the rectal insufflation
is to stop the bleeding associated
with "Bleeding Colitis". In this case, the German standards
indicate that the concentration of ozone should be 60ug/ml and
the amount of ozone infused should only be 60cc. This higher
ozone concentration will stop the bleeding, but will not heal
the colon. Therefore the goal is to first stop the bleeding with
the higher concentration (60ug/ml) and once stopped, the technique
above is used to heal the affected parts of the colon. The basic
guidelines are that ozone at higher concentrations (60 ug/ml)
are used to stop bleeding and to sterilize. After this is accomplished
the ozone concentration should be lowered to the levels known
to cause healing, that is, 30 - 45 ug/ml.
(7) Can I
do this for a longer period of time to increase the effectiveness?
NO. A recent publication encouraging a "45 minute rectal
insufflation" should never be attempted. The author of this
technique is under the impression that "more is better" and
therefore performing an insufflation for this length of time
should be preferable to the short rectal insufflation. Unfortunately
the person who devised this technique and published it in a book
is not a doctor, and has no previous medical training. He therefore
has no training or knowledge with regards to how damaging this
45 minute rectal insufflation would be to people. The guidelines
instruct someone to use two catheters, one deep inside the colon,
and one only inserted a few inches. One catheter is used to infuse
the ozone, while the other is used to allow the ozone to escape.
This prevents the build up of any pressure within the colon,
and allows the user to infuse ozone for as long as desired. Again,
this method is assuming that 'more is better'. Unfortunately
this logic is flawed and has absolutely no scientific basis.
There is no increase in benefit in using this method, and in
fact, the user attempting this method runs a very clear risk
of massive irritation of the sensitive tissue within the colon.
Ozone in proper amounts is a wonderful healing tool, however
attempting a 45 minute rectal insufflation can actually cause
irritation and damage of this sensitive tissue.
of a steam sauna on the immune system and disease processes
have been well documented. In addition to being relaxing and
soothing, a sauna and the accompanying induced hyperthermia
on the body, mimics the beneficial effects of a fever, without
the discomfort. At 104 degrees F., for example, the growth
rate of the polio virus is reduced up to 250 times; at 106
degrees pneumococcus, a bacterium responsible for pneumonia,
the effects of this artificial method of increasing the body
temperature are not as comprehensive as a natural fever, there
are definite system wide effects. There is evidence that artificial
fever works as an immune system stimulant by increasing the
number of white blood cells in the body. In a 1959-review of
studies on the effects of heat treatments, Mayo Clinic researcher
Dr. Wakim and colleagues cite findings indicating that the
number of white blood cells in the blood increased by an average
of 58% during artificially induced fever. Researchers also
have found increases in the activity of the white blood cells
during induced fever.
the immune system-stimulating effects of sweat therapy, many
thought it as one of the most effective and painless detoxifying
treatments available. Sweat contains almost the same elements
as urine, and for this reason, the skin is sometimes called
the third kidney. It is estimated that as much as 30% of bodily
wastes are eliminated by way of perspiration, and during a
steam sauna, the body perspires profusely.
ozone into this environment, the ozone is easily absorbed into
the skin and lymphatic system because of the open pores. This
provides an excellent detoxifying effect and it is also reported
that the effects are once again not limited to detoxification,
but may induce the desirable system wide effects that is desired
in the medical use of ozone: oxygenation of all the tissues,
organs, and cells of the body, boosting the immune system,
stimulation of the release of anti-cancer substances from the
white blood cells of the immune system, enhancement of blood
and oxygen delivery throughout the body.
It is very
important to point out that the sauna used for this treatment
is of the type where the individual's head protrudes from the
top of the sauna, and the individual is therefore not breathing
the ozone / steam mixture. Concentrations used for this type
of treatment are usually approximately 30 - 40ug/ml, and the
ozone is introduced into the sauna at a rate of 1/4LPM.
steam sauna is one of the most popular methods of taking advantage
of this therapy. The steam sauna ozone system for applying
ozone has many advantages:
It is a relaxing
and non-invasive approach to ozone therapy
It cleanses the skin, pores, and lymphatic system
The user will take advantage of 2 therapies at the same time: sweat therapy
and ozone therapy (both individually with their own health benefits)
You feel clean, refreshed, and rejuvenated.
have found it beneficial to irrigate the ear canal with the
ozone / oxygen mixture from a medical ozone generator. As with
every use of ozone for medical purposes the individual must
ensure: the purity of the oxygen supply, that all parts of
the ozone generator in contact with the gas stream are ozone
resistant (eg. kynar, Teflon, glass, silicone), and that they
know the ozone output (concentration of the ozone) of the ozone
generator. This is extremely important - too little ozone and
there will be no effect; too much ozone and there can be irritation
to the ear canal.
may first apply a small amount of water to the ear canal to
assist in the absorption of the ozone. The ozone must be humidified
by bubbling the gas through water. The usual concentration
used for this protocol is 15ug/ml - 30ug/ml. The flow rate
used is usually ¼ Litre per minute or perhaps 1/8 LPM.
tube of the ozone generator is held up to the entrance of the
ear. At no time should the tube come into proximity of the
eyes, nose, or mouth. Doing so may cause discomfort. At no
time should the output tube be placed inside the ear, or sealed
into the entrance way of the ear.
The ozone should be allowed to flow into the ear and out of the ear. Each ear
is usually treated for 2-5 minutes per treatment.
schedule depends on the disease of the patient, and the effect
on each individual patient. This therapy should not be performed
more than once per day, and in most cases is performed 2-3
times per week.
should individually modify the treatment protocol depending
on their reaction. If any discomfort, redness, or "chapping" occurs,
it is suggested that the patient increase the length of time
between treatments, and decrease the ozone concentration coming
from the ozone generator.
have indicated that the ozone enters the lymphatic and blood
system this way. Many patients have reported a wide variety
of results including relief of allergies, relief of colds,
clearing of sore throats and swollen glands.
cannula is used to introduce the ozone gas to the patient.
Unlike Rectal Insufflation, there is no danger of pressure
buildup. The ozone concentration used is usually 25-30ug/ml,
and the flow rate is usually 1/4LPM (litres per minute) or
1/8LPM. Treatment time is usually 5 - 15 minutes. The patient
may lower the ozone concentration, and the duration of the
treatment if any discomfort occurs. This treatment should not
be performed more than once per day, and is usually only performed
2-3 times per week.
have reported relief from yeast infections and various sexually
transmitted diseases including herpes. Many women also use
this method as an alternative to rectal insufflation as it
is theorized that the ozone not only affects the pelvic region,
but also enters the general circulation causing a body wide
not use this method close to, or during the time of menstruation
as ozone at these concentrations increases blood flow.
One of the
easiest and most pleasurable of the methods of using ozone,
the body suit is both effective and relaxing. The ozone is
absorbed through the skin, cleansing the lymphatic system,
and reportedly inducing the body wide effects of ozone generally
seen with other treatment protocols.
first opens the pores of the skin by taking a warm/hot shower,
after which they immediately enter the body suit.
The body suit must be sealed at the ankles and wrists to reduce leaking. Usually
a towel is wrapped around the neck to increase comfort and to reduce leaking
around the neck. If necessary, a fan may be used to gently blow any leaking
ozone away from the individual. The ozone generator is set to produce a concentration
of 25 - 35ug/ml at a flow rate of 1/4LPM.
The ozone must be humidified (run through water) and is introduced into the
remains in the body suit for 15 - 40 minutes ( one usually
starts with a 15 minute treatment and the treatment time remains
that or is gradually increased depending on the desire and
comfort of the individual during and after treatments). Treatment
is usually individualized depending on the individual effects
of the treatments and the desired effect, be it in treatment
of a disease, or for general health. Individuals should be
encouraged to use their own bodies and common sense as an indication
of whether treatment times and concentrations should be increased
or lowered. This treatment is usually performed 2-3 times per
week, and never more than once per day.
Olive Oil is used around the world for a variety of uses: acne,
skin lesions, burns, fungal infections (eg. Of the toe nail),
herpes, eczema, leg sores, bed sores, gingivitis, hemorrhoids,
STD's, cold sores, and many others.
to be the only way to stabilize ozone without adding artificial
stabilizers, chemicals, or preservatives. Ozone is bubbled
at very high concentrations, under a controlled environment
for days until it slowly begins to solidify. This solid form
of olive oil forms a Vaseline or salve like substance and will
keep for many months on the shelf. If kept refrigerated, it
maintains its full effectiveness almost indefinitely.
even choose to ingest ozonated olive oil in order to obtain
the system wide effects of using ozone medically. Click here
for instructions as to how to make ozonated olive oil.
ozone refers to the method of isolating a body part by surrounding
it with a bag (such as hand, arm, leg, foot, torso, pelvis,
but NEVER head), and introducing ozone. This can be used to
treat gangrene, diabetic foot ulcers, bed sores, burns, any
wounds that are infected or slow healing, or those that refuse
to heal. This method is not intended to generate an immune
modulating effect on the individual as in the methods above.
The bag is
placed around or over the affected area, the output tube from
the ozone generator placed through the top of the bag, and
the top sealed as effectively as possible. Ozone at the desired
concentration must first be humidified (simply bubbled through
water) and then enters the bag; the ozone generator constantly
runs during this treatment. Treatment times vary depending
on the type of wounds being treated but typically range from
10 - 30 minutes. As leaking will occur from the bag it is suggested
that this process occurs in a well ventilated area. After the
treatment the bag will still contain a high concentration of
ozone, irritating to the lungs, nose, and eyes if it escapes
from the bag. This is usually disposed of in a safe manner.
(In European medical clinics, a specially designed bag is used
to ensure no leaking occurs, and after treatment the ozone
is sucked from the bag by a vacuum pump so neither the patient,
nor the attendant ever breathes in the ozone).
In this method
the ozone kills any bacteria, viruses, fungus, or molds infecting
the open wound, increase blood flow to the wound, and stimulates
the healing process. It has been documented that many a body
part has been spared amputation through the application of
ozone in this method.
of ozone applied through bagging (60ug/ml - 90ug/ml) tend to
have a sterilization effect on the wound, but if used for prolonged
periods will have a negative effect on healing. Mid range ozone
concentrations (30-40 ug/ml) will have a healing effect on
the wounds. Therefore the protocol as suggested by German researchers
is to begin with the infected wound at 75-90ug/ml for the sterilizing
effect, and as the treatments begin to clear the wound of infection,
to gradually drop the concentration towards 35ug/ml. This gradual
drop in the concentration will maintain the sterility of the
wound, and stimulate healing. Individual differences in this
protocol are taken into account by watching the effect of the
ozone on the wound, and the rate at which it closes.
is performed only by trained professionals, usually only in
a clinic setting. Major Autohemotherapy
is the preferred method of many practitioners, and it is used
in well over 150 diseases. In Germany, even the ambulances
are equipped with ozone generators; if ozone is administered
within 24 hours of a stroke, 95% of patients suffer no permanent
obviously requires medical grade ozone with no contaminants.
Ozone generators that produce ozone from 'air' should never be used for this protocol, even if they have been fitted with
an oxygen tank. Only ozone generators designed to produce pure
medical ozone should be used. This protocol requires standard
blood administration equipment, such as a Blood Administration
evacuated container (or empty saline bag), heparin, calcium,
and 60 cc syringes. The procedure involves the removal of approximately
125 to 200cc of the patients blood. This blood is withdrawn
into the evacuated bottle or into an empty saline bag. The
bottle (or bag) is then hung upside down, and ozone gas (the
volume as the
withdrawn) is infused into the blood. But at what concentration?
Velio Bocci of Italy can be accredited with being a major influence
the world of ozone in that he has been researching ozone for
over 30 years at the University of Sienna. His research has
that greatest immune system stimulation (activation) effects
occur when the ozone is introduced into the blood at a concentration
His detailed research has displayed that this concentration
of 78 ug/ml illicites the maximum cytokine production from
the white blood cells.
These cytokines enter the body when the blood is reintroduced
to the patient, and in turn stimulate other white blood cells
within the body to also release cytokines, inducing an immunostimulating
effect that spreads through the body like a chain reaction.
While lower concentrations of ozone can be used for Major Autohemotherapy,
Bocci's research clearly showed that cytokine production is
maximized when the ozone is bubbled through the blood at 78
In this procedure
the trained professional constantly monitors the patient, and
ensures the purity of the ozone gas, and the sterility of all
equipment used. For the trained professional, this treatment
is actually quite easy, and very safe for the patient.